Mass-Spectrometry Based Proteome Comparison of Extracellular Vesicle Isolation Methods: Comparison of ME-kit, Size-Exclusion Chromatography, and High-Speed Centrifugation

Mass-Spectrometry Based Proteome Comparison of Extracellular Vesicle Isolation Methods: Comparison of ME-kit, Size-Exclusion Chromatography, and High-Speed Centrifugation

Extracellular vesicles (EVs) are small membrane-enclosed particles released by cells under various conditions specific to cells’ biological states. Hence, mass-spectrometry (MS) based proteome analysis of EVs in plasma has gained much attention as a method to discover novel protein biomarkers. MS an...

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Bibliographic Details
Main Authors: Anders Askeland, Anne Borup, Ole Østergaard, Jesper V. Olsen, Sigrid M. Lund, Gunna Christiansen, Søren R. Kristensen, Niels H. H. Heegaard, Shona Pedersen
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Biomedicines
Subjects:
Extracellular vesicles
human plasma
EV isolation
high-speed centrifugation
size exclusion chromatography
ME kit
Online Access:https://www.mdpi.com/2227-9059/8/8/246

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https://www.mdpi.com/2227-9059/8/8/246

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