A Novel Complementation Assay for Quick and Specific Screen of Genes Encoding Glycerol-3-Phosphate Acyltransferases

A Novel Complementation Assay for Quick and Specific Screen of Genes Encoding Glycerol-3-Phosphate Acyltransferases

The initial step in glycerolipid biosynthesis, especially in diverse allopolyploid crop species, is poorly understood, mainly due to the lack of an effective and convenient method for functional characterization of genes encoding glycerol-3-phosphate acyltransferases (GPATs) catalyzing this reaction...

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Bibliographic Details
Main Authors: Jie Lei, Yingchun Miao, Yu Lan, Xiuxiu Han, Hongbo Liu, Yi Gan, Leilei Niu, Yanyan Wang, Zhifu Zheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-03-01
Series:Frontiers in Plant Science
Subjects:
glycerolipid
phosphatidic acid
glycerol-3-phosphate acyltransferase
yeast complementation
heterologous expression
Arabidopsis
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00353/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2018.00353/full

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