A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA Assembly.

A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA Assembly.

RNA interference (RNAi) denotes sequence-specific mRNA degradation induced by short interfering double-stranded RNA (siRNA) and has become a revolutionary tool for functional annotation of mammalian genes, as well as for development of novel therapeutics. The practical applications of RNAi are usual...

Full description

Bibliographic Details
Main Authors: Fang Deng, Xiang Chen, Zhan Liao, Zhengjian Yan, Zhongliang Wang, Youlin Deng, Qian Zhang, Zhonglin Zhang, Jixing Ye, Min Qiao, Ruifang Li, Sahitya Denduluri, Jing Wang, Qiang Wei, Melissa Li, Nisha Geng, Lianggong Zhao, Guolin Zhou, Penghui Zhang, Hue H Luu, Rex C Haydon, Russell R Reid, Tian Yang, Tong-Chuan He
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4232585?pdf=render

Internet

http://europepmc.org/articles/PMC4232585?pdf=render

Similar Items