A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR
<p>Abstract</p> <p>Background</p> <p>Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construction of target-specific s...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2008-05-01
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Series: | BMC Biotechnology |
Online Access: | http://www.biomedcentral.com/1472-6750/8/47 |