Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at differe...

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Bibliographic Details
Main Authors: Luca Lanzanò, Lorenzo Scipioni, Melody Di Bona, Paolo Bianchini, Ranieri Bizzarri, Francesco Cardarelli, Alberto Diaspro, Giuseppe Vicidomini
Format: Article
Language:English
Published: Nature Publishing Group 2017-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00117-2

Internet

https://doi.org/10.1038/s41467-017-00117-2

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