OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of <i>Octopus vulgaris</i>

• Main Authors: Valeria Maselli, Emilia Galdiero, Anna Maria Salzano, Andrea Scaloni, Angela Maione, Annarita Falanga, Daniele Naviglio, Marco Guida, Anna Di Cosmo, Stefania Galdiero
• Format: Article
• Language: English
• Published: MDPI AG 2020-07-01
• Series: Marine Drugs
• Subjects: <i>Octopus vulgaris</i>; antimicrobial peptides; antimicrobial activity; antibiofilm activity
• Online Access: https://www.mdpi.com/1660-3397/18/8/380
• ISSN: 1660-3397

Microorganism resistance to conventional antibiotics represents one of the major global health concerns. This paper focuses on a peptide (OctoPartenopin) extracted from suckers of <i>Octopus vulgaris;</i> bioassay-guided chromatographic fractionation was used to identify this sequence, which holds significant antibacterial activity against Gram-positive and Gram-negative bacteria. OctoPartenopin is encrypted within the calponin sequence and was associated with the high levels of proteolytic activity already reported in octopus arm suckers. We synthesized the parent peptide and four analogues; all peptide were tested for their antibacterial and antibiofilm activities. Preliminary antibiofilm experiments showed that that one of the analogues had the best activity in both inhibition and eradication of biofilm of all three microorganisms tested. The occurrence of OctoPartenopin in arm suckers provided novel speculative information on animal behavior, as concerns maternal care of fertilized eggs. Our results highlight that suckers are a rich source of multifaceted peptides to develop alternative antimicrobial agents and food preservatives.