An improved method for rapid isolation of DNA and RNA from leaves, flowers and roots of blackgram [Vigna mungo (L.) Hepper] for detection of begomovirus infection and RT-PCR.
Blackgram is an important pulse crop grown for its protein rich seeds. Its tissues are rich in polyphenols which hampered pure nucleic acid extraction and thus use of molecular techniques in breeding programme. In this study, a well established DNA extraction method for legumes, Gem-CTAB method has...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Society of Plant Breeders
2019-03-01
|
| Series: | Electronic Journal of Plant Breeding |
| Subjects: | |
| Online Access: | http://ejplantbreeding.org/index.php/EJPB/article/view/3122 |