Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to deter...

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Bibliographic Details
Main Authors: Ricardo D. Righetto, Nikhil Biyani, Julia Kowal, Mohamed Chami, Henning Stahlberg
Format: Article
Language:English
Published: Nature Publishing Group 2019-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09661-5