Dedicated Setup for the Photoconversion of Fluorescent Dyes for Functional Electron Microscopy

Dedicated Setup for the Photoconversion of Fluorescent Dyes for Functional Electron Microscopy

Here, we describe a cost-effective setup for targeted photoconversion of fluorescent signals into electron dense ones. This approach has offered invaluable insights in the morphology and function of fine neuronal structures. The technique relies on the localized oxidation of diaminobenzidine (DAB) m...

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Bibliographic Details
Main Authors: Katharine L. Dobson, Carmel L. Howe, Yuri Nishimura, Vincenzo Marra
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-07-01
Series:Frontiers in Cellular Neuroscience
Subjects:
FM1-43
Lucifer Yellow
synaptic vesicle (SV)
functional labeling
electron microscopy
CLEM
Online Access:https://www.frontiersin.org/article/10.3389/fncel.2019.00312/full

Internet

https://www.frontiersin.org/article/10.3389/fncel.2019.00312/full

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