Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion prot...

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Bibliographic Details
Main Authors: Mehdi Imani, Hossein Zarei Jaliani, Mohammad Hassan Kheirandish, Mahnaz Azadpour
Format: Article
Language:English
Published: Mashhad University of Medical Sciences 2017-04-01
Series:Iranian Journal of Basic Medical Sciences
Subjects:
Online Access:http://ijbms.mums.ac.ir/article_8577_824c161b1816e4d3be8871c0d3bdfef3.pdf