Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants

• Main Authors: Nouf Mohamed Al-Rasheed, Laila Mohamed Fadda, Nawal Mohamed Al-Rasheed, Hanaa Mahmoud Ali, Hazar Ibrahim Yacoub
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar)
• Series: Brazilian Archives of Biology and Technology
• Subjects: CCl4; Silymarin; Chlorogenic acid; Melatonin; mRNA expression
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100408&lng=en&tlng=en

The objective of this study is to examine whether silymarin alone or in combination with chlorogenic acid and/ or melatonin plays a modulatory role against apoptotic damage in rats liver induced by of CCl4. The present work revealed that CCl4 induced elevation of in Bax, Smad, TGF-β and NFkBhepatic mRNA expression, administration of silymarin alone down regulates these expressions. Treatment with chlorogenic acid and/ or melatonin along with silymarin produced best results in this concern. Bcl-2 expression was down regulated by CCl4 whereas concurrent treatment of chlorogenic acid and/ or melatonin along with silymarin increased this expression. On conclusion, the use of chlorogenic acid and/ or melatonin potentiates the anti-apoptotic action of silymarin.