Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing
Summary: The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during se...
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Elsevier
2018-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718300755 |
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doaj-6f5d4b3086434e71a0af795210bf9f012020-11-25T03:34:14ZengElsevierCell Reports2211-12472018-02-012261589159910.1016/j.celrep.2018.01.043Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA SequencingIsabelle Stévant0Yasmine Neirijnck1Christelle Borel2Jessica Escoffier3Lee B. Smith4Stylianos E. Antonarakis5Emmanouil T. Dermitzakis6Serge Nef7Department of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland; SIB, Swiss Institute of Bioinformatics, University of Geneva, 1211 Geneva, SwitzerlandDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, SwitzerlandDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, SwitzerlandDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, SwitzerlandMRC Centre for Reproductive Health, University of Edinburgh, Edinburgh EH16 4TJ, UK; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, AustraliaDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, SwitzerlandDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland; SIB, Swiss Institute of Bioinformatics, University of Geneva, 1211 Geneva, SwitzerlandDepartment of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland; Corresponding authorSummary: The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during sex determination, we identified a single population of somatic progenitor cells prior to sex determination. A subset of these progenitors differentiates into Sertoli cells, a process characterized by a highly dynamic genetic program consisting of sequential waves of gene expression. Another subset of multipotent cells maintains their progenitor state but undergoes significant transcriptional changes restricting their competence toward a steroidogenic fate required for the differentiation of fetal Leydig cells. Our findings confirm the presence of a unique multipotent progenitor population in the gonadal primordium that gives rise to both supporting and interstitial lineages. These also provide the most granular analysis of the transcriptional events occurring during testicular cell-fate commitment.http://www.sciencedirect.com/science/article/pii/S2211124718300755single-cell RNA-seqsex determinationtestisSertoli cellfetal Leydig cellprogenitors |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Isabelle Stévant Yasmine Neirijnck Christelle Borel Jessica Escoffier Lee B. Smith Stylianos E. Antonarakis Emmanouil T. Dermitzakis Serge Nef |
| spellingShingle |
Isabelle Stévant Yasmine Neirijnck Christelle Borel Jessica Escoffier Lee B. Smith Stylianos E. Antonarakis Emmanouil T. Dermitzakis Serge Nef Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing Cell Reports single-cell RNA-seq sex determination testis Sertoli cell fetal Leydig cell progenitors |
| author_facet |
Isabelle Stévant Yasmine Neirijnck Christelle Borel Jessica Escoffier Lee B. Smith Stylianos E. Antonarakis Emmanouil T. Dermitzakis Serge Nef |
| author_sort |
Isabelle Stévant |
| title |
Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing |
| title_short |
Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing |
| title_full |
Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing |
| title_fullStr |
Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing |
| title_full_unstemmed |
Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing |
| title_sort |
deciphering cell lineage specification during male sex determination with single-cell rna sequencing |
| publisher |
Elsevier |
| series |
Cell Reports |
| issn |
2211-1247 |
| publishDate |
2018-02-01 |
| description |
Summary: The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during sex determination, we identified a single population of somatic progenitor cells prior to sex determination. A subset of these progenitors differentiates into Sertoli cells, a process characterized by a highly dynamic genetic program consisting of sequential waves of gene expression. Another subset of multipotent cells maintains their progenitor state but undergoes significant transcriptional changes restricting their competence toward a steroidogenic fate required for the differentiation of fetal Leydig cells. Our findings confirm the presence of a unique multipotent progenitor population in the gonadal primordium that gives rise to both supporting and interstitial lineages. These also provide the most granular analysis of the transcriptional events occurring during testicular cell-fate commitment. |
| topic |
single-cell RNA-seq sex determination testis Sertoli cell fetal Leydig cell progenitors |
| url |
http://www.sciencedirect.com/science/article/pii/S2211124718300755 |
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