Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins

Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins

The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the exis...

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Bibliographic Details
Main Authors: Rosilene Oliveira Mesquita, Eduardo de Almeida Soares, Everaldo Gonçalves de Barros, Marcelo Ehlers Loureiro
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2012-01-01
Series:Genetics and Molecular Biology
Subjects:
proteomics
Glycine max
2-DE
protein extraction
leaf proteome
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200017

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200017

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