Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker

Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker

Fish express two different chitinases, acidic fish chitinase-1 (AFCase-1) and acidic fish chitinase-2 (AFCase-2), in the stomach. AFCase-1 and AFCase-2 have different degradation patterns, as fish efficiently degrade chitin ingested as food. For a comparison with the enzymatic properties and the pri...

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Bibliographic Details
Main Authors: Satoshi Kawashima, Hiroki Ikehata, Chihiro Tada, Tomohiro Ogino, Hiromi Kakizaki, Mana Ikeda, Hideto Fukushima, Masahiro Matsumiya
Format: Article
Language:English
Published: MDPI AG 2016-01-01
Series:Marine Drugs
Subjects:
fish stomach chitinase isozymes
cDNA cloning
Japanese sardine Sardinops melanostictus
fish-specific acidic fish chitinase
long linker
Online Access:http://www.mdpi.com/1660-3397/14/1/22

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http://www.mdpi.com/1660-3397/14/1/22

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