Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content

Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content

Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are mediators of cell survival and pathogenesis by facilitating virulence factor dissemination and resistance to antimicrobials. Studies of OMV properties often focus on hypervesiculating Escherichia coli mutants that have increased O...

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Bibliographic Details
Main Authors: Shelby L. Reimer, Daniel R. Beniac, Shannon L. Hiebert, Timothy F. Booth, Patrick M. Chong, Garrett R. Westmacott, George G. Zhanel, Denice C. Bay
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Microbiology
Subjects:
outer membrane vesicles
Escherichia coli
ultracentrifugation
ultradiafiltration
hypervesiculation
Tol-Pal system
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.628801/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.628801/full

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