True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.

Bibliographic Details
Main Authors: Christophe Jung, Peter Bandilla, Marc von Reutern, Max Schnepf, Susanne Rieder, Ulrich Unnerstall, Ulrike Gaul
Format: Article
Language:English
Published: Nature Publishing Group 2018-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03977-4
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spelling doaj-6de05cf9c14c415c94eeb469a7b935652021-05-11T10:00:28ZengNature Publishing GroupNature Communications2041-17232018-04-019111110.1038/s41467-018-03977-4True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopyChristophe Jung0Peter Bandilla1Marc von Reutern2Max Schnepf3Susanne Rieder4Ulrich Unnerstall5Ulrike Gaul6Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenGene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität MünchenMethods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.https://doi.org/10.1038/s41467-018-03977-4
collection DOAJ
language English
format Article
sources DOAJ
author Christophe Jung
Peter Bandilla
Marc von Reutern
Max Schnepf
Susanne Rieder
Ulrich Unnerstall
Ulrike Gaul
spellingShingle Christophe Jung
Peter Bandilla
Marc von Reutern
Max Schnepf
Susanne Rieder
Ulrich Unnerstall
Ulrike Gaul
True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
Nature Communications
author_facet Christophe Jung
Peter Bandilla
Marc von Reutern
Max Schnepf
Susanne Rieder
Ulrich Unnerstall
Ulrike Gaul
author_sort Christophe Jung
title True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_short True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_full True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_fullStr True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_full_unstemmed True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_sort true equilibrium measurement of transcription factor-dna binding affinities using automated polarization microscopy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-04-01
description Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.
url https://doi.org/10.1038/s41467-018-03977-4
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AT ulrichunnerstall trueequilibriummeasurementoftranscriptionfactordnabindingaffinitiesusingautomatedpolarizationmicroscopy
AT ulrikegaul trueequilibriummeasurementoftranscriptionfactordnabindingaffinitiesusingautomatedpolarizationmicroscopy
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