True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-03977-4 |