Cloning a Chymotrypsin-Like 1 (CTRL-1) Protease cDNA from the Jellyfish Nemopilema nomurai

Cloning a Chymotrypsin-Like 1 (CTRL-1) Protease cDNA from the Jellyfish Nemopilema nomurai

An enzyme in a nematocyst extract of the Nemopilema nomurai jellyfish, caught off the coast of the Republic of Korea, catalyzed the cleavage of chymotrypsin substrate in an amidolytic kinetic assay, and this activity was inhibited by the serine protease inhibitor, phenylmethanesulfonyl fluoride. We...

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Bibliographic Details
Main Authors: Yunwi Heo, Young Chul Kwon, Seong Kyeong Bae, Duhyeon Hwang, Hye Ryeon Yang, Indu Choudhary, Hyunkyoung Lee, Seungshic Yum, Kyoungsoon Shin, Won Duk Yoon, Changkeun Kang, Euikyung Kim
Format: Article
Language:English
Published: MDPI AG 2016-07-01
Series:Toxins
Subjects:
Nemopilema nomurai
amidolytic kinetic assay
cloning a chymotrypsin-like 1 (CTRL-1) protease
full-length cDNA sequence
genomic DNA sequence
Online Access:http://www.mdpi.com/2072-6651/8/7/205

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http://www.mdpi.com/2072-6651/8/7/205

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