Compartment-specific pH monitoring in Bacillus subtilis using fluorescent sensor proteins; a tool to analyse the antibacterial effect of weak organic acids.
The internal pH (pHi) of a living cell is one of its most important physiological parameters. To monitor the pH inside B. subtilis during various stages of its life cycle, we constructed an improved version (IpHluorin) of the ratiometric, pH-sensitive fluorescent protein pHluorin by extending it at...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00157/full |