Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler

Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler

We developed a simple and cheap assay for quantitatively detecting ochratoxin A (OTA) in wine. A DNA aptamer available in literature was used as recognition probe in its molecular beacon form, i.e., with a fluorescence-quenching pair at the stem ends. Our aptabeacon could adopt a conformation allo...

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Bibliographic Details
Main Authors: Simona Marianna Sanzani, Massimo Reverberi, Corrado Fanelli, Antonio Ippolito
Format: Article
Language:English
Published: MDPI AG 2015-03-01
Series:Toxins
Subjects:
OTA detection
fluorescent dyes
aptabeacon
Real-time PCR thermal cycler
food safety
Online Access:http://www.mdpi.com/2072-6651/7/3/812

Internet

http://www.mdpi.com/2072-6651/7/3/812

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