Effect of mutations on the thermostability of Aspergillus aculeatus β-1,4-galactanase

Effect of mutations on the thermostability of Aspergillus aculeatus β-1,4-galactanase

New variants of β-1,4-galactanase from the mesophilic organism Aspergillus aculeatus were designed using the structure of β-1,4-galactanase from the thermophile organism Myceliophthora thermophila as a template. Some of the variants were generated using PROPKA 3.0, a validated pKa prediction tool, t...

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Bibliographic Details
Main Authors: Søs Torpenholt, Leonardo De Maria, Mats H.M. Olsson, Lars H. Christensen, Michael Skjøt, Peter Westh, Jan H. Jensen, Leila Lo Leggio
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
β-1,4-galactanase
Thermostability
Computational prediction
Protein design
GH53
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037015000197

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http://www.sciencedirect.com/science/article/pii/S2001037015000197

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