Time saving method for protoplast isolation, transformation and transient gene expression assay in barley
This study was conducted to establish a rapid method for barley (Hordeum vulgare L.) protoplast isolation to provide an easy-to-use procedure for the transformation and primary investigation of new gene constructs by transient gene expression assays. Protoplasts were successfully isolated from the c...
Main Authors: | Barnabas Jenes, Matti Puolimatka, Pedro Bittencourt, Seppo Pulli |
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Format: | Article |
Language: | English |
Published: |
Scientific Agricultural Society of Finland
1994-03-01
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Series: | Agricultural and Food Science |
Online Access: | https://journal.fi/afs/article/view/72695 |
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