Time saving method for protoplast isolation, transformation and transient gene expression assay in barley

This study was conducted to establish a rapid method for barley (Hordeum vulgare L.) protoplast isolation to provide an easy-to-use procedure for the transformation and primary investigation of new gene constructs by transient gene expression assays. Protoplasts were successfully isolated from the c...

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Bibliographic Details
Main Authors: Barnabas Jenes, Matti Puolimatka, Pedro Bittencourt, Seppo Pulli
Format: Article
Language:English
Published: Scientific Agricultural Society of Finland 1994-03-01
Series:Agricultural and Food Science
Online Access:https://journal.fi/afs/article/view/72695