Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Human papillomaviruses (HPVs) are small DNA tumor viruses that mainly infect mucosal epithelia of anogenital and upper respiratory tracts. There has been progressive demand for more analytical assays for HPV DNA quantification. A novel droplet digital PCR (ddPCR) method was developed to simultaneous...

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Bibliographic Details
Main Authors: John Charles Rotondo, Lucia Oton-Gonzalez, Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Mauro Tognon, Fernanda Martini
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Microbiology
Subjects:
droplet digital PCR
ddPCR
quantitative PCR
human papillomavirus
viral DNA load
cervical intraepithelial neoplasia
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.591452/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2020.591452/full

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