Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.

Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.

Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an efficient procedure for making precise alterations to...

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Bibliographic Details
Main Authors: Yen Kuan Ng, Muhammad Ehsaan, Sheryl Philip, Mark M Collery, Clare Janoir, Anne Collignon, Stephen T Cartman, Nigel P Minton
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3566075?pdf=render

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http://europepmc.org/articles/PMC3566075?pdf=render

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