A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function

Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-te...

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Bibliographic Details
Main Authors: Christopher J. Perry, Eleanor C. Warren, Joseph L. Damstra-Oddy, Claire Storey, Lisa M. Francione, Sarah J. Annesley, Paul R. Fisher, Annette Müller-Taubenberger, Robin S.B. Williams
Format: Article
Language:English
Published: Elsevier 2020-07-01
Series:Biochemistry and Biophysics Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580820300601