Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

Abstract Objective We aim to directly detect Mycoplasma DNA in a U937 suspension cell culture without using DNA purification. In order to make Mycoplasma contamination monitoring easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit. We compared the sensitivity of...

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Bibliographic Details
Main Authors: Zain Baaity, Sven Breunig, Kamil Önder, Ferenc Somogyvári
Format: Article
Language:English
Published: BMC 2019-11-01
Series:BMC Research Notes
Subjects:
Mycoplasma
qPCR
PCR
Direct
Elimination
Online Access:http://link.springer.com/article/10.1186/s13104-019-4763-5

Internet

http://link.springer.com/article/10.1186/s13104-019-4763-5

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