Generation of a single chain antibody variable fragment (scFv) to sense selectively RhoB activation.

Generation of a single chain antibody variable fragment (scFv) to sense selectively RhoB activation.

Determining the cellular level of activated form of RhoGTPases is of key importance to understand their regulatory functions in cell physiopathology. We previously reported scFvC1, that selectively bind to the GTP-bound form of RhoA, RhoB and RhoC. In this present study we generate, by molecular evo...

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Bibliographic Details
Main Authors: Patrick Chinestra, Aurélien Olichon, Claire Medale-Giamarchi, Isabelle Lajoie-Mazenc, Rémi Gence, Cyril Inard, Laetitia Ligat, Jean-Charles Faye, Gilles Favre
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4218836?pdf=render

Internet

http://europepmc.org/articles/PMC4218836?pdf=render

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