A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse glioma

A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse glioma

Abstract Molecular biomarkers provide both diagnostic and prognostic results for patients with diffuse glioma, the most common primary brain tumor in adults. Here, we used a long-read nanopore-based sequencing technique to simultaneously assess IDH mutation status and MGMT methylation level in 4 hum...

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Bibliographic Details
Main Authors: Thidathip Wongsurawat, Piroon Jenjaroenpun, Annick De Loose, Duah Alkam, David W. Ussery, Intawat Nookaew, Yuet-Kin Leung, Shuk-Mei Ho, John D. Day, Analiz Rodriguez
Format: Article
Language:English
Published: BMC 2020-06-01
Series:Acta Neuropathologica Communications
Subjects:
Glioblastoma
MGMT
IDH
Targeted sequencing
CRISPR/Cas9
Long-read sequencing
Online Access:http://link.springer.com/article/10.1186/s40478-020-00963-0

Internet

http://link.springer.com/article/10.1186/s40478-020-00963-0

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