Development of real-time PCR assay allowed describing the first clinical Klebsiella pneumoniae isolate harboring plasmid-mediated colistin resistance mcr-8 gene in Algeria

Development of real-time PCR assay allowed describing the first clinical Klebsiella pneumoniae isolate harboring plasmid-mediated colistin resistance mcr-8 gene in Algeria

Objectives: We aimed to develop here a specific real-time PCR assay with TaqMan® probe to detect efficiently bacterial strains harboring the new plasmid mediated-colistin resistance mcr-8 gene. Methods: Specific primers and probe for mcr-8 gene were designed from sequences alignment of all mcr genes...

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Bibliographic Details
Main Authors: Larbi Zakaria Nabti, Farida Sahli, Edgarthe Priscilla Ngaiganam, Nadia Radji, Wahiba Mezaghcha, David Lupande-Mwenebitu, Sophie Alexandra Baron, Jean-Marc Rolain, Seydina M. Diene
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Journal of Global Antimicrobial Resistance
Subjects:
Colistin resistance
mcr-8 gene
Real-time-PCR assay
Diagnostic tool
TaqMan probe
Online Access:http://www.sciencedirect.com/science/article/pii/S2213716519302164

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http://www.sciencedirect.com/science/article/pii/S2213716519302164

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