Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases

Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases

The trans-2-deoxyribosylation of 4-thiouracil (4SUra) and 2-thiouracil (2SUra), as well as 6-azauracil, 6-azathymine and 6-aza-2-thiothymine was studied using dG and E. coli purine nucleoside phosphorylase (PNP) for the in situ generation of 2-deoxy-α-D-ribofuranose-1-phosphate (dRib-1P) followed by...

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Bibliographic Details
Main Authors: Vladimir A. Stepchenko, Anatoly I. Miroshnikov, Frank Seela, Igor A. Mikhailopulo
Format: Article
Language:English
Published: Beilstein-Institut 2016-12-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
enzymatic glycosylation
PM3 and ab initio calculations
recombinant E. coli uridine, thymidine and purine nucleoside phosphorylases
substrate properties
4(2)-thioxo- and 6(5)-aza-uacil and -thymine
Online Access:https://doi.org/10.3762/bjoc.12.254

Internet

https://doi.org/10.3762/bjoc.12.254

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