A de novo Full-Length mRNA Transcriptome Generated From Hybrid-Corrected PacBio Long-Reads Improves the Transcript Annotation and Identifies Thousands of Novel Splice Variants in Atlantic Salmon

A de novo Full-Length mRNA Transcriptome Generated From Hybrid-Corrected PacBio Long-Reads Improves the Transcript Annotation and Identifies Thousands of Novel Splice Variants in Atlantic Salmon

Atlantic salmon (Salmo salar) is a major species produced in world aquaculture and an important vertebrate model organism for studying the process of rediploidization following whole genome duplication events (Ss4R, 80 mya). The current Salmo salar transcriptome is largely generated from genome sequ...

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Bibliographic Details
Main Authors: Sigmund Ramberg, Bjørn Høyheim, Tone-Kari Knutsdatter Østbye, Rune Andreassen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Genetics
Subjects:
Atlantic salmon
transcriptome
full-length mRNA
hybrid error correction
PacBio Iso-seq
Illumina sequencing
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.656334/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2021.656334/full

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