Rapid detection of MDR–Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens

Rapid detection of MDR–Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens

Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant based on polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique that analyzes the katG, rpoB genes. Methods: Biochemical test as well as IS6110 targeting PCR revealed 1...

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Bibliographic Details
Main Authors: Imani Fooladi Abbas Ali, Farzam Babak, Mousavi Seyed Fazlollah, Jonaidi Jafari Nematollah
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2014-05-01
Series:Asian Pacific Journal of Tropical Biomedicine
Subjects:
Drug resistance
Mycobacterium tuberculosis
PCR-SSCP
Phenotypic method
rpoB
katG
Online Access:http://www.sciencedirect.com/science/article/pii/S2221169115302628

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http://www.sciencedirect.com/science/article/pii/S2221169115302628

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