Elimination of silica gel from gangliosides by using a reversed-phase column after preparative thin-layer chromatography.

A simple and effective procedure has been developed for eliminating silica gel from gangliosides after preparative thin-layer chromatography. Gangliosides were extracted from the scraped silica gel with chloroform-methanol-water 10:10:3 (by volume) and dried. The residue was suspended in 0.1 M NaCl...

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Bibliographic Details
Main Authors: H Kubo, M Hoshi
Format: Article
Language:English
Published: Elsevier 1985-05-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520343534