New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species

New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species

A highly efficient method for chromosomal integration of cloned DNA into Methanosarcina spp. was developed utilizing the site-specific recombination system from the Streptomyces phage φC31. Host strains expressing the φC31 integrase gene and carrying an appropriate recombination site can be transfor...

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Bibliographic Details
Main Authors:	Adam M. Guss, Michael Rother, Jun Kai Zhang, Gargi Kulkkarni, William W. Metcalf
Format:	Article
Language:	English
Published:	Hindawi Limited 2008-01-01
Series:	Archaea
Online Access:	http://dx.doi.org/10.1155/2008/534081

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