New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species
A highly efficient method for chromosomal integration of cloned DNA into Methanosarcina spp. was developed utilizing the site-specific recombination system from the Streptomyces phage φC31. Host strains expressing the φC31 integrase gene and carrying an appropriate recombination site can be transfor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2008-01-01
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| Series: | Archaea |
| Online Access: | http://dx.doi.org/10.1155/2008/534081 |