Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing

Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing

Summary: Translation regulation is a fundamental step in gene regulation with critical roles in neurodevelopment. Here, we describe three protocols to calculate the ribosomal-engagement levels of the transcriptome from in vitro-derived neuronal cells. The protocols described here include enrichment...

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Bibliographic Details
Main Authors: Deivid Carvalho Rodrigues, Marat Mufteev, James Ellis
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Sequence analysis
Sequencing
RNA-seq
Gene Expression
Neuroscience
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302161

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http://www.sciencedirect.com/science/article/pii/S2666166720302161

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