Reproducibility across single-cell RNA-seq protocols for spatial ordering analysis.

As newer single-cell protocols generate increasingly more cells at reduced sequencing depths, the value of a higher read depth may be overlooked. Using data from three different single-cell RNA-seq protocols that lend themselves to having either higher read depth (Smart-seq) or many cells (MARS-seq...

Full description

Bibliographic Details
Main Authors: Morten Seirup, Li-Fang Chu, Srikumar Sengupta, Ning Leng, Hadley Browder, Kevin Kapadia, Christina M Shafer, Bret Duffin, Angela L Elwell, Jennifer M Bolin, Scott Swanson, Ron Stewart, Christina Kendziorski, James A Thomson, Rhonda Bacher
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0239711