Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

Abstract ω-Transaminases’ (ω-TAs) importance for synthesizing chiral amines led to the development of different methods to quickly identify and characterize new sources of these enzymes. Here we describe the optimization of growth and induction of such an enzyme in a wild type strain of Bacillus sp....

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Bibliographic Details
Main Authors: Najme Gord Noshahri, Jamshid Fooladi, Ulrike Engel, Delphine Muller, Michaela Kugel, Pascal Gorenflo, Christoph Syldatk, Jens Rudat
Format: Article
Language:English
Published: SpringerOpen 2021-03-01
Series:AMB Express
Subjects:
ω-Transaminase
ortho-Xylylenediamine assay
Native PAGE
Activity staining
Growth optimization
Bacillus fermentation
Online Access:https://doi.org/10.1186/s13568-021-01207-7

Internet

https://doi.org/10.1186/s13568-021-01207-7

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