A novel selection marker for efficient DNA cloning and recombineering in E. coli.

Production of recombinant DNA in bacterial cells is an essential technique in molecular biology. Plasmids are usually maintained in an E. coli host by antibiotic selection. However, there are only a few antibiotic-resistance markers available in common use. Here we report the adoption of a novel sel...

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Bibliographic Details
Main Authors: Chuan-Wei Jang, Terry Magnuson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3577784?pdf=render