Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody.

Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody.

GFP-tagged proteins are used extensively as biosensors for protein localization and function, but the GFP moiety can interfere with protein properties. An alternative is to indirectly label proteins using intracellular recombinant antibodies (scFvs), but most antibody fragments are insoluble in the...

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Bibliographic Details
Main Authors: Lynne Cassimeris, Laurence Guglielmi, Vincent Denis, Christian Larroque, Pierre Martineau
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555790/pdf/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555790/pdf/?tool=EBI

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