High-throughput construction of intron-containing hairpin RNA vectors for RNAi in plants.

With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for making hairpin RNA (hpRNA) constructs is in great demand. Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction o...

Full description

Bibliographic Details
Main Authors: Pu Yan, Wentao Shen, XinZheng Gao, Xiaoying Li, Peng Zhou, Jun Duan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3364983?pdf=render