CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
The ability to target Cas9 to specific genomic loci offers the potential for applications beyond genome editing. Here the authors use deactivated Cas9 and chimeric guide RNAs to visualise individual repetitive loci in living cells.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11707 |