Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System

Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System

Constrained peptides are an attractive class as affinity reagents or drug leads owing to their excellent binding properties. Many kinds of these peptides, such as cyclic peptides containing disulfide bridges, are found in nature or designed artificially by directed evolution. However, confirming the...

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Bibliographic Details
Main Authors: Yutaro Tanemura, Yuki Mochizuki, Shigefumi Kumachi, Naoto Nemoto
Format: Article
Language:English
Published: MDPI AG 2015-03-01
Series:Biology
Subjects:
molecular interaction analysis
pull-down assay
cell-free translation system
puromycin
constrained peptide
cyclic peptide
disulfide-rich peptide
cross-linking
in vitro selection
directed evolution
Online Access:http://www.mdpi.com/2079-7737/4/1/161

Internet

http://www.mdpi.com/2079-7737/4/1/161

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