Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease Digestion

Large regions of nearly identical repeats, such as the 45S ribosomal RNA (rRNA) genes of Nucleolus Organizer Regions (NORs), can account for major gaps in sequenced genomes. To assemble these regions, ultra-long sequencing reads that span multiple repeats have the potential to reveal sets of repeats...

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Bibliographic Details
Main Authors: Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S. Pikaard
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Plant Science
Subjects:
NOR
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.656049/full