Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants

Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants

Gene targeting (GT) enables precise genome modification—e.g., the introduction of base substitutions—using donor DNA as a template. Combined with clean excision of the selection marker used to select GT cells, GT is expected to become a standard, generally applicable, base editing system. Previously...

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Bibliographic Details
Main Authors: Namie Ohtsuki, Keiko Kizawa, Akiko Mori, Ayako Nishizawa-Yokoi, Takao Komatsuda, Hitoshi Yoshida, Katsuyuki Hayakawa, Seiichi Toki, Hiroaki Saika
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Genome Editing
Subjects:
gene targeting
precise genome modification
single-strand annealing
cleistogamy 1
oryza sativa
miRNA target site
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2020.617713/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2020.617713/full

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