The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ di...

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Bibliographic Details
Main Authors: Weihua Tian, Zilu Ye, Shengjun Wang, Morten Alder Schulz, Julie Van Coillie, Lingbo Sun, Yen-Hsi Chen, Yoshiki Narimatsu, Lars Hansen, Claus Kristensen, Ulla Mandel, Eric Paul Bennett, Siamak Jabbarzadeh-Tabrizi, Raphael Schiffmann, Jin-Song Shen, Sergey Y. Vakhrushev, Henrik Clausen, Zhang Yang
Format: Article
Language:English
Published: Nature Publishing Group 2019-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09809-3

Internet

https://doi.org/10.1038/s41467-019-09809-3

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