Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons

Summary: Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of...

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Bibliographic Details
Main Authors: Ji Won Um, Kyung Ah Han, Se-Young Choi, Jaewon Ko
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300824