Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

Abstract Background To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide base...

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Bibliographic Details
Main Authors: Muhammad Waqar Hameed, Ilona Juszczak, Ralph Bock, Joost Thomas van Dongen
Format: Article
Language:English
Published: BMC 2017-12-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-017-0257-4