Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues
Abstract Background To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide base...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2017-12-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13007-017-0257-4 |