Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Our understanding of the genus Natrinema is presently limited due to the lack of available genetic tools. Auxotrophic markers have been widely used to construct genetic systems in bacteria and eukaryotes and in some archaeal species. Here, we isolated four auxotrophic mutants of Natrinema sp. J7-2,...

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Bibliographic Details
Main Authors: Jie Lv, Shuai Wang, Yuchen Wang, Yuping Huang, Xiangdong Chen
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Archaea
Online Access:http://dx.doi.org/10.1155/2015/483194